In this paper, the advantages of the Shewanella mixed culture system in terms of carbon source utilization range, pollutant degradation performance, and bioelectricity generation performance were reviewed. The impacts of environmental conditions and strain types on the Shewanella mixed culture system were systematically analyzed, the mechanism of action of the Shewanella mixed bacterial system was comprehensively summarized, and the research progress in its applications such as environmental pollution remediation and bioelectricity generation was thoroughly presented. The aim is to provide support for further systematic research and practical application of the Shewanella mixed culture system.