This paper is oriented to the talent cultivation needs of singing art majors, observes the application effect of professional vocal training programs, and explores the path and influence mechanism of its effect on singers’ artistic expression. The experimental samples are selected and experimental and control classes are set. In the experimental class, melodic dictation and sight-singing are the main training contents, and the selection of teaching materials, training methods and practice methods are based on the training objectives. Under this professional vocal training mode, the students in the experimental class were able to actively use the professional vocal techniques of vibrato, straight voice and jittery voice, and they also had a high level of singing breath and vocalization (>50.00%) and vocal discrimination (>83.33%), and 73.33% of the students showed a high level of singing self-confidence and singing preference. The experimental results show that professional vocal training is not only limited to the improvement of singing skills and abilities, but also creates a good learning atmosphere, enhances the motivation and interest of the singers, and improves the artistic expression of the singers through the combination of external performance and internal perception.